microarray slide hybridization (OakLabs Inc)
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Microarray Slide Hybridization, supplied by OakLabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+slide+hybridization/microarray+analysis/pm26991510-89-9-12
Average 90 stars, based on 1 article reviews
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Microarray:Article Title: Shoot tolerance mechanisms to iron toxicity in rice (Oryza sativa L.). Article Snippet: Iron toxicity frequently affects lowland rice and leads to oxidative stress via the Fenton reaction.. Tolerance mechanisms were investigated in contrasting genotypes: the intolerant IR29 and the tolerant recombinant inbred line FL483.. Seedlings were exposed to 1000mgL ferrous iron, and the regulation of genes involved in three hypothetical tolerance mechanisms was investigated (I) Iron uptake, partitioning and storage. Article Title: Genetic factors underlying boron toxicity tolerance in rice: genome-wide association study and transcriptomic analysis Article Snippet: RNA samples were first analyzed regarding purity (Nanodrop 2000c, Thermo Fisher Scientific Inc., Wilmington, DE, USA) and integrity (Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). .. Only samples with RNA integrity number >8 were used for Hybridization:Article Title: Shoot tolerance mechanisms to iron toxicity in rice (Oryza sativa L.). Article Snippet: Iron toxicity frequently affects lowland rice and leads to oxidative stress via the Fenton reaction.. Tolerance mechanisms were investigated in contrasting genotypes: the intolerant IR29 and the tolerant recombinant inbred line FL483.. Seedlings were exposed to 1000mgL ferrous iron, and the regulation of genes involved in three hypothetical tolerance mechanisms was investigated (I) Iron uptake, partitioning and storage. Article Title: Genetic factors underlying boron toxicity tolerance in rice: genome-wide association study and transcriptomic analysis Article Snippet: RNA samples were first analyzed regarding purity (Nanodrop 2000c, Thermo Fisher Scientific Inc., Wilmington, DE, USA) and integrity (Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). .. Only samples with RNA integrity number >8 were used for |
![(A) HIF-1α, HIF-2α, PHD2, and PHD3 protein levels were analyzed after cultivating BMDM for 24 h at 20% or 1% O2. (B) The expression of HIF-1 target genes, such as PKM2, Pfk, and PDK-1, on mRNA level was analyzed after cultivating BMDM for 24 h at 20% O2 or 1% O2 using qRT-PCR. (C) PHD3−/− [knockout (KO)] and WT BMDM were cultivated for 48 h at 20% O2 and 1% O2. Subsequently, cells were lysed and RNA isolated. RNA samples were analyzed in a transcriptome <t>microarray</t> assay. The volcano blots were generated based on the microarray results. (D) PHD3−/− and WT BMDM were cultured with or without serum for 24 h or stimulated with 100 ng LPS for 12 h. Subsequently, NF-κB activities were analyzed. As a positive control for the NF-κB activity assay, a cell extract of HeLa cells, which were treated with LPS, was used; n = 3; mean ± sd; **P < 0.01, and ***P < 0.001. rel. LU, Relative light units.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5934/pmc05395934/pmc05395934__zgb0071461060002.jpg)